Terminator

Part:BBa_K3296007:Design

Designed by: Shiyuan Li   Group: iGEM19_Shanghai_HS   (2019-10-15)


B0015 terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The mfold results are annotated with the location of the subparts BBa_B0010 and BBa_B0012 and the BioBrick assembly scar. Double terminator consisting of BBa_B0010 and BBa_B0012.This is the most commonly used terminator. It seems to be reliable. However, that Part:BBa_B0014 is a better part for forward and reverse termination.


Source

Artificial synthesis.

References